Lab report on isolation of microorganism from soil

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Lab report on isolation of microorganism from soil

Akbar Haqi Introduction Microorganism is an organism that is microscopic or submicroscopic, which is too small to be seen under naked eyes. However, the numbers of microorganisms in a given sample are required to know in certain aspect such as dairy industries, diseases investigation, and so on.

Because of this, a variety of methods have been developed for the enumeration of microorganisms like direct microscopic counts, filtration and viable plate counts Jacquelyn G. Among the methods of enumeration, viable plate counts are being used most frequently to measure bacterial populations.

In viable plate counts, we are measuring the number of viable cells, unlike the microscopic counts which cannot distinguish live from dead cells. However, it takes some time for the visible colonies to grow Gerard J.

Lab report on isolation of microorganism from soil

Before doing plate counts, serial dilutions are required. This is because it is hard to count more than colonies on an agar plate if we inoculated directly from the original bacterial suspension or sample without serial dilutions Kathleen Talaro, To complete plate counts, we could either use pour plate method or spread plate method and each of them have their advantages and limitations.

Introduction

All the visible colonies are calculated and represented as colony forming units CFU. Then, the CFU is multiplied with the corresponding dilution factor.

As a result, the population of original sample is known. The objectives of this experiment are to learn the process of enumeration to determine the number of microorganisms in a sample and utilize two methods in enumeration which are pour plate method and spread plate method.

Pour Plate Method The pour plate, like other viable plate count methods, involves adding a sample to a solid medium that will support microbial growth incubating the plates so that each bacterial cell multiplies to form a colony, and counting the number of colonies that develop.

Generally we have no idea of the number of bacteria in a sample, so it is almost always necessary to prepare a dilution series to ensure that you will obtain a dilution containing a reasonable number of bacteria to count.

Six nutrient agar pours are melted in a boiling water bath. Three 99 mL dilution blanks are labeled as 10 -2,and respectively. Six Petri plates are labeled through The unknown sample is shaken to ensure an even distribution of microorganisms generally shaking side to side for 25 times.

The dilution blank is shaken vigorously to distribute the bacteria evenly. Using a new sterile pipette, 0. With the same pipette, an additional 1. The original 1 mL of sample has now been diluted 1 part in a total of 10, parts.

The shaking procedure is repeated for the 10 -4 blank and 0.

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This same procedure is repeated to form a 10 -6 dilution blank from which you will establish 10 -7 0. The plate is swirled to mix the sample with the agar. The agar is made sure does not run over the edges of the plate. The lid is replaced. The agar is allowed to cool and solidity.

After incubation, the colonies are counted on each plate. Both the colonies on the agar surface and the colonies growing within the agar must be counted. The colonies are counted by marking their position on the back of the Petri plates with a marking pen. This aid in keeping track of those colonies previously counted and avoids recounts.

If a plate has more than colonies record it as TNTC too numerous to count. From the plate-count data, the concentration of bacteria in the original sample is calculated. For statistical reasons only plates with between 30 and colonies are used in this calculation.

Each colony forming unit CFU represents the progeny of a single cell. Therefore, the number of bacterial cells in the original sample is determined by multiplying the number of colonies on a dilution plate by the corresponding dilution factor.ISOLATION OF SOIL BACTERIA: VIABLE TITER and PURE CULTURE.

bacteria in soil an overestimate or underestimate of the actual number of viable bacteria in soil? 3. How would you design an experiment to test the answers to Question 2? Daily Lab Report: Soil Titer and Pure culture - 1 Name: RESULTS.

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LAB REPORT OF MICROBIOLOGY. Uploaded by. Akbar Haqi. Introduction Microorganism is an organism that is microscopic or submicroscopic, which is too small to be seen under naked eyes.

However, the numbers of microorganisms in a given sample are required to know in certain aspect such as dairy industries, diseases investigation, and so on. Lab 2 - Microbial Enumeration Some former study found that bacteria in soil solution has a higher total counts than in pure culture [2].

However, an opposite result was found in this who will use them to do the DNA extraction for the DNA gel electrophoresis. Does extra virgin olive oil have the same adverse effect on arterial function as refined oils and animal fats?

Lab Report On Isolation Of Microorganism From Soil LAB Report #3 Introduction: In this lab we have focus on Isolation of bacteria from environment. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes, walls, furniture; in soil and dust; and on and in our own bodies (skin and .

LAB Report #3 Introduction: In this lab we have focus on Isolation of bacteria from environment. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes, walls, furniture; in soil and dust; and on and in our own bodies (skin and mucous membranes).

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